{"id":22065,"date":"2021-03-24T07:57:00","date_gmt":"2021-03-24T06:57:00","guid":{"rendered":"https:\/\/acva.sk\/evaluation-of-16s-rrna-primer-sets-for-characterisation-of-microbiota-in-paediatric-patients-with-autism-spectrum-disorder\/"},"modified":"2022-08-22T12:52:57","modified_gmt":"2022-08-22T10:52:57","slug":"evaluation-of-16s-rrna-primer-sets-for-characterisation-of-microbiota-in-paediatric-patients-with-autism-spectrum-disorder","status":"publish","type":"post","link":"https:\/\/acva.sk\/en\/evaluation-of-16s-rrna-primer-sets-for-characterisation-of-microbiota-in-paediatric-patients-with-autism-spectrum-disorder\/","title":{"rendered":"Evaluation of 16S rRNA primer sets for characterisation of microbiota in paediatric patients with autism spectrum disorder"},"content":{"rendered":"<p>AUTHORS:&nbsp;<span dir=\"ltr\" role=\"presentation\">Palkova<\/span><span dir=\"ltr\" role=\"presentation\">, L.<\/span> <span dir=\"ltr\" role=\"presentation\">, <\/span><span dir=\"ltr\" role=\"presentation\">Tomova<\/span><span dir=\"ltr\" role=\"presentation\">, A., <\/span><span dir=\"ltr\" role=\"presentation\">Repiska<\/span><span dir=\"ltr\" role=\"presentation\">, G., <\/span><span dir=\"ltr\" role=\"presentation\">Babinska<\/span>, K., <span dir=\"ltr\" role=\"presentation\">Bokor<\/span><span dir=\"ltr\" role=\"presentation\">, B., <\/span><span dir=\"ltr\" role=\"presentation\">Mikula<\/span><span dir=\"ltr\" role=\"presentation\">, I.,<\/span><span dir=\"ltr\" role=\"presentation\">Minarik<\/span><span dir=\"ltr\" role=\"presentation\">, G.,<\/span> <span dir=\"ltr\" role=\"presentation\">Ostatnikova<\/span><span dir=\"ltr\" role=\"presentation\">, D. and<\/span> <span dir=\"ltr\" role=\"presentation\">Soltys. K.<\/span><\/p>\n<p>ABSTRACT: I<span dir=\"ltr\" role=\"presentation\">ntestinal microbiota is becoming a significant marker that reflects differences between <\/span><span dir=\"ltr\" role=\"presentation\">health and disease status also in terms of gut-brain axis communication. Studies show that children <\/span><span dir=\"ltr\" role=\"presentation\">with autism spectrum disorder (ASD) often have a mix of gut microbes that is distinct from the <\/span><span dir=\"ltr\" role=\"presentation\">neurotypical children. Various assays are being used for microbiota investigation and were considered <\/span><span dir=\"ltr\" role=\"presentation\">to be universal. However, newer studies showed that protocol for preparing DNA sequencing libraries <\/span><span dir=\"ltr\" role=\"presentation\">is a key factor influencing results of microbiota investigation. The choice of DNA amplification <\/span><span dir=\"ltr\" role=\"presentation\">primers seems to be the crucial for the outcome of analysis. In our study, we have tested 3 primer <\/span><span dir=\"ltr\" role=\"presentation\">sets to investigate differences in outcome of sequencing analysis of microbiota in children with <\/span><span dir=\"ltr\" role=\"presentation\">ASD. We found out that primers detected different portion of bacteria in samples especially at <\/span><span dir=\"ltr\" role=\"presentation\">phylum level; significantly higher abundance of Bacteroides and lower Firmicutes were detected <\/span><span dir=\"ltr\" role=\"presentation\">using 515f\/806r compared to 27f\/1492r and 27f*\/1495f primers. So, the question is whether a gold <\/span><span dir=\"ltr\" role=\"presentation\">standard of Firmicutes\/Bacteroidetes ratio is a valuable and reliable universal marker, since two primer <\/span><span dir=\"ltr\" role=\"presentation\">sets towards 16S rRNA can provide opposite information. Moreover, significantly higher relative <\/span><span dir=\"ltr\" role=\"presentation\">abundance of Proteobacteria was detected using 27f\/1492r. The beta diversity of sample groups <\/span><span dir=\"ltr\" role=\"presentation\">differed remarkably and so the number of observed bacterial genera.<\/span><\/p>\n<p>Scientific Reports 11<b data-test=\"journal-volume\"><\/b>, Article&nbsp;number:&nbsp;<span data-test=\"article-number\">6781<\/span> (<span data-test=\"article-publication-year\">2021<\/span>)<\/p>\n<p><a href=\"https:\/\/doi.org\/10.1038\/s41598-021-86378-w\">https:\/\/doi.org\/10.1038\/s41598-021-86378-w<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>AUTHORS:&nbsp;Palkova, L. , Tomova, A., Repiska, G., Babinska, K., Bokor, B., Mikula, I.,Minarik, G., Ostatnikova, D. and Soltys. K. ABSTRACT: Intestinal microbiota is becoming a significant marker that reflects differences between health and disease status also in terms of gut-brain axis communication. Studies show that children with autism spectrum disorder (ASD) often have a mix<\/p>\n","protected":false},"author":5,"featured_media":21920,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_eb_attr":"","footnotes":""},"categories":[341,107],"tags":[167,357,355,356],"class_list":["post-22065","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-2021-en","category-publications","tag-autism-spectrum-disorder-en","tag-gut-brain-axis-en","tag-intestinal-microbiota-en","tag-primer-en"],"_links":{"self":[{"href":"https:\/\/acva.sk\/en\/wp-json\/wp\/v2\/posts\/22065","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/acva.sk\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/acva.sk\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/acva.sk\/en\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/acva.sk\/en\/wp-json\/wp\/v2\/comments?post=22065"}],"version-history":[{"count":1,"href":"https:\/\/acva.sk\/en\/wp-json\/wp\/v2\/posts\/22065\/revisions"}],"predecessor-version":[{"id":23038,"href":"https:\/\/acva.sk\/en\/wp-json\/wp\/v2\/posts\/22065\/revisions\/23038"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/acva.sk\/en\/wp-json\/wp\/v2\/media\/21920"}],"wp:attachment":[{"href":"https:\/\/acva.sk\/en\/wp-json\/wp\/v2\/media?parent=22065"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/acva.sk\/en\/wp-json\/wp\/v2\/categories?post=22065"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/acva.sk\/en\/wp-json\/wp\/v2\/tags?post=22065"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}